3U2V
Crystal Structure of Human Glycogenin-1 (GYG1) complexed with manganese, UDP and maltohexaose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-08-11 |
| Detector | ADSC Q315 3x3 CCD |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 |
| Unit cell lengths | 46.679, 46.739, 69.690 |
| Unit cell angles | 79.56, 88.71, 76.86 |
Refinement procedure
| Resolution | 44.760 - 1.500 |
| R-factor | 0.18255 |
| Rwork | 0.181 |
| R-free | 0.21743 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3q4s |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.514 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.450 | 1.580 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.043 | 0.362 |
| Number of reflections | 85751 | |
| <I/σ(I)> | 11.4 | 2.4 |
| Completeness [%] | 94.9 | 92.2 |
| Redundancy | 2.4 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293.15 | 2.0M Ammonium Sulphate, 0.1M HEPES, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293.15K |
