3U2U
Crystal Structure of Human Glycogenin-1 (GYG1) complexed with manganese, UDP and maltotetraose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-06 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 1 |
| Unit cell lengths | 46.765, 46.844, 69.848 |
| Unit cell angles | 79.36, 88.45, 77.20 |
Refinement procedure
| Resolution | 41.200 - 1.450 |
| R-factor | 0.16533 |
| Rwork | 0.164 |
| R-free | 0.19330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3q4s |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.526 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.600 | 1.530 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.043 | 0.425 |
| Number of reflections | 95223 | |
| <I/σ(I)> | 15.7 | 2.8 |
| Completeness [%] | 94.5 | 91.6 |
| Redundancy | 3.7 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293.15 | 2.0M Ammonium Sulphate, 0.2M Na/K tartrate, 0.1M Na Citrate, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 293.15K |
