3U2P

Crystal structure of N-terminal three extracellular domains of ErbB4/Her4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E+ SUPERBRIGHT
Temperature [K]	100
Detector technology	CCD
Collection date	2009-09-30
Detector	RIGAKU SATURN 944+
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	42.170, 85.560, 152.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.870 - 2.570
R-factor	0.1951
Rwork	0.192
R-free	0.24290
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ahx
RMSD bond length	0.010
RMSD bond angle	1.240
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	BUSTER (2.8.0)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.660
High resolution limit [Å]	2.570	5.530	2.570
Rmerge	0.089	0.061	0.472
Number of reflections	17809
<I/σ(I)>	10.2
Completeness [%]	97.1	98.3	97.2
Redundancy	5.2	5.7	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	16% PEG8000,0.2M Calcium acetate, 0.1M Socium Cacodylate, VAPOR DIFFUSION, HANGING DROP, temperature 293K, pH 6.0