3U2M
Crystal structure of human ALR mutant C142/145S
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OXFORD DIFFRACTION ENHANCE ULTRA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-06 |
| Detector | OXFORD ONYX CCD |
| Wavelength(s) | 1.541 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 50.400, 76.880, 62.460 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.940 - 2.000 |
| R-factor | 0.23559 |
| Rwork | 0.230 |
| R-free | 0.29122 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3o55 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.736 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.090 | 0.500 |
| Number of reflections | 8795 | |
| <I/σ(I)> | 7.1 | 1.8 |
| Completeness [%] | 93.6 | 93.1 |
| Redundancy | 4.5 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 298 | 0.1 M MES, 20% PEG6000 , pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
