3U2B

Structure of the Sox4 HMG domain bound to DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2011-06-20
Detector	ADSC QUANTUM 315
Wavelength(s)	1.075
Spacegroup name	P 32 2 1
Unit cell lengths	69.941, 69.941, 63.205
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.732 - 2.402
R-factor	0.2377
Rwork	0.235
R-free	0.27950
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.698
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASES
Refinement software	PHENIX ((phenix.refine: 1.5_2))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.400
Number of reflections	7040
Completeness [%]	98.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2	298	100mM Hepes pH 7.2, 20% PEG 3350 pH 7.2, 50mM MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 298K