3U2A
Adaptor dependent degradation of a cell-cycle regulator reveals diversity in substrate architectures
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | OTHER |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-01-31 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 63.799, 41.895, 48.659 |
| Unit cell angles | 90.00, 107.52, 90.00 |
Refinement procedure
| Resolution | 46.400 - 1.700 |
| R-factor | 0.1693 |
| Rwork | 0.167 |
| R-free | 0.20617 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.238 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 3.660 | 1.700 |
| Rmerge | 0.050 | 0.030 | 0.271 |
| Number of reflections | 11481 | ||
| <I/σ(I)> | 20.7 | ||
| Completeness [%] | 84.1 | 98.4 | 30.9 |
| Redundancy | 6.7 | 6.8 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 0.1 M Sodium Acetate, 8% (w/v) PEG 4000, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
