3U27
Crystal structure of ethanolamine utilization protein EutL from Leptotrichia buccalis C-1013-b
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-06-10 |
| Detector | SBC-3 |
| Wavelength(s) | 0.97920 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 100.854, 105.644, 101.551 |
| Unit cell angles | 90.00, 90.06, 90.00 |
Refinement procedure
| Resolution | 34.417 - 1.852 |
| R-factor | 0.1584 |
| Rwork | 0.157 |
| R-free | 0.18180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.089 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MLPHARE |
| Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.000 | 1.880 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Number of reflections | 176454 | |
| <I/σ(I)> | 14.58 | 2.28 |
| Completeness [%] | 99.6 | 99.8 |
| Redundancy | 4.1 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 4M Na Nitrate, 0.1M BistrisPropane 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298 K |
