3U26
Crystal Structure of Engineered Protein. Northeast Structural Genomics Consortium Target OR48
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-08-10 |
| Detector | ADSC QUANTUM 4r |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 33.606, 67.579, 48.193 |
| Unit cell angles | 90.00, 109.30, 90.00 |
Refinement procedure
| Resolution | 45.490 - 1.590 |
| R-factor | 0.20245 |
| Rwork | 0.200 |
| R-free | 0.24803 |
| Structure solution method | SAD |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.177 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.660 |
| High resolution limit [Å] | 1.590 | 1.600 |
| Rmerge | 0.040 | 0.128 |
| Number of reflections | 27144 | |
| Completeness [%] | 91.0 | 69 |
| Redundancy | 2 | 1.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 0.1M PBS buffer pH 7.5 25% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
