3U1R
Structure Analysis of A New Psychrophilic Marine Protease
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-BM |
| Synchrotron site | APS |
| Beamline | 17-BM |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2007-12-01 |
| Detector | ADSC QUANTUM 4r |
| Wavelength(s) | 1.1000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 45.907, 75.195, 115.416 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.000 |
| R-factor | 0.16399 |
| Rwork | 0.161 |
| R-free | 0.21152 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.968 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.052 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 25533 | |
| Completeness [%] | 91.0 | 63.44 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 25% PEG 4K, 0.1 M Tris pH 8.0, 0.2 M sodium acetate, 0.1 M Li2SO4, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
