3U1A
N-terminal 81-aa fragment of smooth muscle tropomyosin alpha
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-11-08 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 58.768, 63.181, 101.299 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.519 - 2.000 |
| R-factor | 0.2245 |
| Rwork | 0.222 |
| R-free | 0.26520 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.935 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX (Autosol) |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.106 | 0.420 |
| Number of reflections | 25949 | |
| <I/σ(I)> | 14.59 | 4.52 |
| Completeness [%] | 98.9 | 98.2 |
| Redundancy | 12.05 | 10.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.4 | 293 | 0.1 M Sodium acetate, pH 4.4, 20 mM Calcium chloride, 30-33 % 2-METHYL-2,4-PENTANEDIOL , VAPOR DIFFUSION, HANGING DROP, temperature 293K |
