3U07
Crystal Structure of the VPA0106 protein from Vibrio parahaemolyticus. Northeast Structural Genomics Consortium Target VpR106.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-09-18 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97898 |
| Spacegroup name | P 1 |
| Unit cell lengths | 51.445, 96.320, 100.540 |
| Unit cell angles | 60.61, 80.87, 86.55 |
Refinement procedure
| Resolution | 46.958 - 2.083 |
| R-factor | 0.195 |
| Rwork | 0.194 |
| R-free | 0.21600 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.403 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXS |
| Refinement software | PHENIX (1.7.1_743) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.180 |
| High resolution limit [Å] | 2.083 | 2.100 |
| Rmerge | 0.048 | 0.205 |
| Number of reflections | 188380 | |
| <I/σ(I)> | 13.3 | 2.8 |
| Completeness [%] | 95.6 | 79.8 |
| Redundancy | 1.4 | 1.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Microbatch crystallization under oil | 6 | 291 | 20% PEG 4000, 0.1M sodium nitrate, 0.1M MES, pH 6.0, Microbatch crystallization under oil, temperature 291K |
