3U06
Crystal structure of the kinesin-14 NcdG347D
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-04-17 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 160.539, 67.094, 94.369 |
| Unit cell angles | 90.00, 98.91, 90.00 |
Refinement procedure
| Resolution | 46.615 - 2.350 |
| R-factor | 0.2174 |
| Rwork | 0.216 |
| R-free | 0.25030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.759 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.7.2_868) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.390 |
| High resolution limit [Å] | 2.350 | 6.370 | 2.350 |
| Rmerge | 0.072 | 0.029 | 0.425 |
| Number of reflections | 36362 | ||
| <I/σ(I)> | 9.5 | 54.62 | 1.92 |
| Completeness [%] | 86.9 | 100 | 59.3 |
| Redundancy | 5.9 | 6 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 288 | Bis-Tris propane, Na2SO4, PEG3350, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 288K |
