3TZT
The structure of a protein in glycosyl transferase family 8 from Anaerococcus prevotii.
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-12-09 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97918, 0.97929 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 42.498, 99.331, 122.794 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.840 - 2.100 |
| R-factor | 0.1617 |
| Rwork | 0.159 |
| R-free | 0.20590 |
| Structure solution method | MAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.590 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.140 |
| High resolution limit [Å] | 2.100 | 5.700 | 2.100 |
| Rmerge | 0.062 | 0.037 | 0.588 |
| Number of reflections | 31606 | ||
| <I/σ(I)> | 10.4 | ||
| Completeness [%] | 99.8 | 97.1 | 99.9 |
| Redundancy | 7.1 | 6.2 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 297 | 0.2M Amonium citrate dibasic, 20% PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 297K |
