Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-03-13 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97944 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 45.982, 45.982, 203.231 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.080 - 2.399 |
| R-factor | 0.2365 |
| Rwork | 0.235 |
| R-free | 0.26110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1x8y |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.473 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.1.4) |
| Refinement software | REFMAC (refmac_5.6.0117) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.490 |
| High resolution limit [Å] | 2.399 | 5.170 | 2.399 |
| Rmerge | 0.068 | 0.030 | 0.507 |
| Number of reflections | 10417 | ||
| <I/σ(I)> | 10 | ||
| Completeness [%] | 98.9 | 98.6 | 98.4 |
| Redundancy | 11.7 | 10.4 | 11.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 25% PEG3350, 0.2M LISO4, 0.1M BIS-TRIS pH 6.5, 0.1M CrCl3, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
