3TY6
ATP-dependent Protease HslV from Bacillus anthracis str. Ames
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-21 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.97924 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 140.255, 127.543, 90.100 |
| Unit cell angles | 90.00, 130.03, 90.00 |
Refinement procedure
| Resolution | 41.076 - 2.498 |
| R-factor | 0.166 |
| Rwork | 0.165 |
| R-free | 0.20400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDBID 2Z3B |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.101 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((phenix.refine: dev_851)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.540 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 41877 | |
| <I/σ(I)> | 16.5 | 2.1 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 4.2 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289 | 0.1 M Tris pH 8.5, 2.5 M Ammonium sulfate, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
