3TXQ
Crystal Structure of phage 44RR small terminase gp16
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-03-06 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 83.013, 106.292, 136.671 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 83.920 - 2.800 |
| R-factor | 0.24223 |
| Rwork | 0.239 |
| R-free | 0.29783 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3zqo |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.808 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 83.920 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.078 | 0.389 |
| Number of reflections | 30459 | |
| <I/σ(I)> | 19.9 | 4.8 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 5.5 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 25% PEG3350, 0.1M HEPES pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
