3TXA
Structural Analysis of Adhesive Tip pilin, GBS104 from Group B Streptococcus agalactiae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-02-11 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 124.585, 124.585, 117.839 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.788 - 2.619 |
| R-factor | 0.20836 |
| Rwork | 0.206 |
| R-free | 0.25552 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3tvy |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.586 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.788 | 2.670 |
| High resolution limit [Å] | 2.619 | 2.619 |
| Number of reflections | 32251 | |
| <I/σ(I)> | 21.9 | 2 |
| Completeness [%] | 100.0 | 99.4 |
| Redundancy | 7.3 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295 | 15% PEG4000, 0.1 M Tris, pH 8.0, 0.2 M lithium sulfate, 10 mM cadmium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
