3TX8

Crystal structure of a succinyl-diaminopimelate desuccinylase (ArgE) from Corynebacterium glutamicum ATCC 13032 at 2.97 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2010-05-13
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91162,0.97919,0.97936
Spacegroup name	P 65 2 2
Unit cell lengths	82.901, 82.901, 364.175
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.543 - 2.972
R-factor	0.2399
Rwork	0.238
R-free	0.25660
Structure solution method	MOLECULAR REPLACEMENT + MAD
Starting model (for MR)	1vgy
RMSD bond length	0.003
RMSD bond angle	0.625
Data reduction software	XDS
Data scaling software	XSCALE (January 30, 2009)
Phasing software	PHASER (2.3.0)
Refinement software	PHENIX (1.7.1)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.543	29.540	3.050
High resolution limit [Å]	2.970	13.290	2.970
Rmerge	0.095	0.031	1.248
Number of reflections	16179	218	1137
<I/σ(I)>	13.1	37.4	1.5
Completeness [%]	99.1	87.9	98.5
Redundancy	4.6	3.3	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.41	293	43.1% polyethylene glycol 400, 0.20M sodium chloride, 0.1M Na/K phosphate pH 6.41, Additive: 0.006 M zinc chloride, nanodrop, VAPOR DIFFUSION, SITTING DROP, temperature 293K