3TWM

Crystal structure of Arabidopsis thaliana FPG

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.3
Synchrotron site	ALS
Beamline	5.0.3
Temperature [K]	100
Detector technology	CCD
Collection date	2009-05-31
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	46.400, 98.570, 181.480
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.958 - 2.800
Rwork	0.225
R-free	0.28690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3twl
RMSD bond length	0.007
RMSD bond angle	1.295
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.12)
Phasing software	PHASER
Refinement software	CNS

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	19.958	19.958	2.950
High resolution limit [Å]	2.800	8.850	2.800
Rmerge		0.059	0.538
Total number of observations		4394	17705
Number of reflections	21150
<I/σ(I)>	11.5	8.6	1.4
Completeness [%]	99.2	91.1	97.6
Redundancy	6.3	6.2	5.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	277	20% (v/v) PEG 2000 MME, 100 mM KBr, and 100 mM sodium acetate pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K