3TW0
Structural Analysis of Adhesive Tip pilin, GBS104 from Group B Streptococcus agalactiae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-04-14 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 53.071, 77.260, 96.402 |
| Unit cell angles | 74.12, 87.25, 89.99 |
Refinement procedure
| Resolution | 46.304 - 2.000 |
| R-factor | 0.19987 |
| Rwork | 0.198 |
| R-free | 0.23068 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3tvy |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.465 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.304 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.070 | 0.290 |
| Number of reflections | 88454 | |
| <I/σ(I)> | 11.5 | 4.2 |
| Completeness [%] | 89.0 | 80.2 |
| Redundancy | 3.6 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | 20% PEG3000, 0.1 M HEPES, 0.2 M NaCl, 10 mM Spermine-4HCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
