3TVA
Crystal Structure of Xylose isomerase domain protein from Planctomyces limnophilus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-08-07 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 41 2 2 |
| Unit cell lengths | 105.324, 105.324, 178.936 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.551 - 2.148 |
| R-factor | 0.169 |
| Rwork | 0.168 |
| R-free | 0.19300 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.251 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((phenix.refine: dev_851)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.190 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Number of reflections | 55639 | |
| <I/σ(I)> | 8.5 | 3.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 9.9 | 10 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 295 | 0.2 M Magnesium Chloride, 0.1 M Sodium Cacodylate:HCl pH 6.5, 10% (w/v) PEG 3000, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
