3TUV
Crystal structure of insulysin with bound ATP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2008-10-27 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9794 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 114.984, 70.660, 114.060 |
| Unit cell angles | 90.00, 92.63, 90.00 |
Refinement procedure
| Resolution | 41.880 - 2.270 |
| R-factor | 0.21975 |
| Rwork | 0.217 |
| R-free | 0.26823 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.927 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.330 |
| High resolution limit [Å] | 2.270 | 2.270 |
| Number of reflections | 36467 | |
| Completeness [%] | 90.7 | 88.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 295 | 20% PEG 4000, 100 mM sodium citrate 100 mM ammonium acetate, 5 mM ATP, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
