3TUU

Structure of dihydrodipicolinate synthase from the common grapevine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	110
Detector technology	CCD
Collection date	2011-01-01
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.000
Spacegroup name	P 1
Unit cell lengths	70.550, 78.900, 135.350
Unit cell angles	93.19, 95.02, 100.61

Refinement procedure

Resolution	57.170 - 2.200
R-factor	0.18205
Rwork	0.180
R-free	0.22745
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.337
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	134.470	2.257
High resolution limit [Å]	2.200	2.200
Number of reflections	133745
Completeness [%]	97.3	96.98

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.2	293	diffracting crystal were obtained by mixing 2 uL of protein solution (10 mg/mL) with 2 uL of reservoir solution: 0.1 M BTP, 0.2 M NaBr, 20 % (w/v) PEG 3350, pH 8.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K