3TUP
Crystal structure of human mitochondrial PheRS complexed with tRNAPhe in the active open state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-04-12 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.93 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 116.041, 116.041, 123.276 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.940 - 3.050 |
| R-factor | 0.245 |
| Rwork | 0.245 |
| R-free | 0.29510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond angle | 1.540 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 38.950 | 38.940 | 3.410 |
| High resolution limit [Å] | 3.050 | 5.820 | 3.050 |
| Number of reflections | 18827 | ||
| Completeness [%] | 99.0 | 97 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | SMALL TUBES | 7 | 292 | 0.2 M ammonium sulfate, 100 mM MES mono-hydrate, pH 7, and 30 % w/v PEG-MME 5,000, SMALL TUBES, temperature 292K |
