3TUO

Crystal structure of N-terminal domain of DNA-binding protein satb1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2009-10-20
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9793
Spacegroup name	P 21 21 21
Unit cell lengths	35.895, 71.027, 153.763
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.235 - 1.697
R-factor	0.1614
Rwork	0.158
R-free	0.21830
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	0.926
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.760
High resolution limit [Å]	1.697	1.700
Rmerge	0.067	0.187
Number of reflections	42418
<I/σ(I)>	24.7	3
Completeness [%]	91.0	68.9
Redundancy	6.2	2.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	15% polyethylene glycol 3350, 0.15M ammonium citrite dibasic, 4% polypropylene glycol P400, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K
1	VAPOR DIFFUSION, SITTING DROP	7	293	15% polyethylene glycol 3350, 0.15M ammonium citrite dibasic, 4% polypropylene glycol P400, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K