3TU9
Crystal structure of rabbit muscle aldolase bound with 5-O-methyl mannitol 1,6-phosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-27 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0809 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 82.669, 103.056, 84.458 |
| Unit cell angles | 90.00, 98.34, 90.00 |
Refinement procedure
| Resolution | 36.973 - 2.090 |
| R-factor | 0.1599 |
| Rwork | 0.156 |
| R-free | 0.20100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zah |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.999 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.6.1_357)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.973 | 2.160 |
| High resolution limit [Å] | 2.090 | 2.090 |
| Rmerge | 0.110 | 0.589 |
| Number of reflections | 82812 | |
| <I/σ(I)> | 12.4 | 2.2 |
| Completeness [%] | 99.9 | 99.6 |
| Redundancy | 3.8 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 17.5% PEG4000, 0.1 M HEPES sodium, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
