3TTY

Crystal structure of beta-galactosidase from Bacillus circulans sp. alkalophilus in complex with galactose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2010-09-17
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97625
Spacegroup name	H 3
Unit cell lengths	228.200, 228.200, 246.800
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.570 - 2.250
R-factor	0.1625
Rwork	0.160
R-free	0.21050
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3tts
RMSD bond length	0.007
RMSD bond angle	1.116
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.6.4_486))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.300
High resolution limit [Å]	2.250	2.250
Number of reflections	225561
Completeness [%]	99.2	97.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	295	6-9% PEG 3350, 0.1M magnesium sulfate, 0.1M bicine, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K