3TTO
Crystal structure of Leuconostoc mesenteroides NRRL B-1299 N-terminally truncated dextransucrase DSR-E in triclinic form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-07-22 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.933 |
| Spacegroup name | P 1 |
| Unit cell lengths | 66.840, 140.040, 155.460 |
| Unit cell angles | 85.36, 90.92, 76.85 |
Refinement procedure
| Resolution | 51.620 - 3.300 |
| R-factor | 0.2274 |
| Rwork | 0.224 |
| R-free | 0.29075 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3klk |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.107 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.620 | 3.480 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Number of reflections | 80648 | |
| <I/σ(I)> | 4.1 | 1.6 |
| Completeness [%] | 97.5 | 97 |
| Redundancy | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 285 | 17% PEG 3350, 0.2 M ammonium iodide, 80 mM ammonium citrate, 2% glycerol, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 285K |
