3TTN
Crystal structures of polyamine receptors SpuD and SpuE from Pseudomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-25 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.97988 |
| Spacegroup name | P 1 |
| Unit cell lengths | 44.329, 44.367, 81.553 |
| Unit cell angles | 91.50, 95.24, 95.23 |
Refinement procedure
| Resolution | 23.070 - 2.000 |
| R-factor | 0.21328 |
| Rwork | 0.212 |
| R-free | 0.24369 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.028 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.4.0077) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.070 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 40146 | |
| Completeness [%] | 96.5 | 87.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 2.6 | 288 | 0.1M citric acid, 28% PEG 6000, 4% Jeffamine M-600, pH 2.6, VAPOR DIFFUSION, HANGING DROP, temperature 288K |
