3TTM
Crystal structure of SpuD in complex with putrescine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-07-07 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97920 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 65.909, 82.862, 128.287 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 69.670 - 2.000 |
| R-factor | 0.21117 |
| Rwork | 0.209 |
| R-free | 0.25520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1a99 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.184 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.4.0077) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 69.670 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 45469 | |
| Completeness [%] | 94.5 | 70.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 288 | 0.1M sodium acetate, 10mM ZnCl2, 28% PEG 3350, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 288K |
