3TTA
Crystal structure of M-PMV DUTPASE relaxed end-product (dUMP) complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | BW7B |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2005-02-01 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 0.8430 |
Spacegroup name | P 63 |
Unit cell lengths | 60.884, 60.884, 63.867 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 2.000 |
R-factor | 0.16473 |
Rwork | 0.162 |
R-free | 0.20407 |
Structure solution method | RIGID BODY REFINEMENT |
Starting model (for MR) | 2d4l |
RMSD bond length | 0.019 |
RMSD bond angle | 1.885 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | REFMAC (5.2.0005) |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.100 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 8979 | |
<I/σ(I)> | 11.7 | 2.6 |
Completeness [%] | 97.8 | 98.7 |
Redundancy | 2.4 | 2.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | PEG 8000, AMMONIUM CHLORIDE, TRIS, PH 8.5 , VAPOR DIFFUSION, HANGING DROP, temperature 293K |