3TT8
Crystal Structure Analysis of Cu Human Insulin Derivative
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0 |
| Spacegroup name | H 3 |
| Unit cell lengths | 81.560, 81.560, 33.747 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.140 - 1.120 |
| R-factor | 0.13478 |
| Rwork | 0.132 |
| R-free | 0.17902 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mso |
| RMSD bond length | 0.028 |
| RMSD bond angle | 2.070 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.1.20) |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.780 | |
| High resolution limit [Å] | 1.120 | |
| Rmerge | 0.048 | |
| Total number of observations | 14922 | |
| Number of reflections | 31826 | |
| <I/σ(I)> | 15.1 | |
| Completeness [%] | 97.9 | |
| Redundancy | 3.6 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 1mM sodium citrate, volume fractions of acetone 10% and 7.5 mM cooper(II) acetate monohydrate, pH 6.4, VAPOR DIFFUSION, HANGING DROP |
