3TT7
Structure of ClpP from Bacillus subtilis in complex with DFP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-07-04 |
| Detector | BRUKER SMART 6500 |
| Wavelength(s) | 0.9000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 122.337, 152.000, 110.067 |
| Unit cell angles | 90.00, 113.21, 90.00 |
Refinement procedure
| Resolution | 37.119 - 2.558 |
| R-factor | 0.2195 |
| Rwork | 0.216 |
| R-free | 0.28000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ktg |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.324 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.7_650) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.590 |
| High resolution limit [Å] | 2.550 | 6.920 | 2.550 |
| Rmerge | 0.063 | 0.031 | 0.455 |
| Number of reflections | 52506 | ||
| <I/σ(I)> | 19 | ||
| Completeness [%] | 88.3 | 88 | 83 |
| Redundancy | 3 | 3.7 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | hanging drop | 5.6 | 295 | 0.1M bicine/Trizma base(pH 8.5), 0.03M magnesium chloride, 0.03M calcium chloride, 10%(w/v) PEG 4000, 20%(v/v) glycerol, hanging drop, temperature 295K |
