3TT2
Crystal Structure of GCN5-related N-Acetyltransferase from Sphaerobacter thermophilus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-12-11 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97912 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.611, 85.515, 98.626 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.310 - 2.730 |
| R-factor | 0.202 |
| Rwork | 0.197 |
| R-free | 0.25000 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.254 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((phenix.refine: dev_851)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.790 |
| High resolution limit [Å] | 2.730 | 2.730 |
| Number of reflections | 11422 | |
| <I/σ(I)> | 9.1 | 2.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.1 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | 1.6 M magnesium sulphate, 0.1 M MES pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
