3TSY

4-Coumaroyl-CoA Ligase::Stilbene Synthase fusion protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2011-03-07
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	P 41 2 2
Unit cell lengths	117.554, 117.554, 243.777
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.100 - 3.100
R-factor	0.179
Rwork	0.177
R-free	0.20800
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.016
RMSD bond angle	1.327
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX (1.7_650)
Refinement software	PHENIX ((phenix.refine: 1.7_650))

Data quality characteristics

	Overall
Low resolution limit [Å]	49.100
High resolution limit [Å]	3.100
Number of reflections	31913
Completeness [%]	100.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	18.5% PEG-3350, 0.24 M potassium citrate, 10 mM sarcosine, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K