3TSL
Crystal structure of M-PMV DUTPASE relaxed end-product (dUMP) complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-01-18 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.8034 |
| Spacegroup name | P 63 |
| Unit cell lengths | 60.833, 60.833, 64.043 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.200 |
| R-factor | 0.19545 |
| Rwork | 0.193 |
| R-free | 0.22942 |
| Structure solution method | RIGID BODY REFINEMENT |
| Starting model (for MR) | 2d4l |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.704 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | REFMAC (5.2.0005) |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.310 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 6238 | |
| <I/σ(I)> | 10.3 | 2.6 |
| Completeness [%] | 90.3 | 90.3 |
| Redundancy | 1.9 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | PEG 8000, AMMONIUM CHLORIDE, TRIS, PH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
