3TSI
Structure of the parainfluenza virus 5 (PIV5) hemagglutinin-neuraminidase (HN) stalk domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 200 |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-04-25 |
| Detector | MAR scanner 300 mm plate |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 101.276, 101.276, 85.713 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.236 - 2.651 |
| R-factor | 0.2037 |
| Rwork | 0.202 |
| R-free | 0.23790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3t1e |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.088 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.3.0) |
| Refinement software | PHENIX (1.7.1_743) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 2.700 |
| High resolution limit [Å] | 2.650 | 7.160 | 2.650 |
| Rmerge | 0.057 | 0.038 | 0.382 |
| Number of reflections | 14956 | ||
| <I/σ(I)> | 15.2 | ||
| Completeness [%] | 99.0 | 82.8 | 100 |
| Redundancy | 5.4 | 4.5 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 4.5% Tascimate, 0.09 M HEPES, 9% PEG MME 5000, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
