3TSA
Spinosyn Rhamnosyltransferase SpnG
Experimental procedure
| Experimental method | MAD |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-05-31 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 |
| Unit cell lengths | 54.160, 57.060, 68.113 |
| Unit cell angles | 81.46, 73.84, 85.95 |
Refinement procedure
| Resolution | 19.370 - 1.700 |
| R-factor | 0.18384 |
| Rwork | 0.181 |
| R-free | 0.23053 |
| Structure solution method | MAD |
| Starting model (for MR) | 2p6p |
| RMSD bond length | 0.025 |
| RMSD bond angle | 2.166 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.410 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 72305 | |
| Completeness [%] | 89.4 | 85.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.9 | 298.15 | 18% w/v PEG3350, 1% v/v glycerol, 13% w/v glucose, 0.1 M sodium cacodylate, pH 6.9, VAPOR DIFFUSION, SITTING DROP, temperature 298.15K |
