3TS5
Crystal Structure of a Light Chain Domain of Scallop Smooth Muscle Myosin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.075 |
| Spacegroup name | P 1 |
| Unit cell lengths | 50.811, 68.775, 79.418 |
| Unit cell angles | 77.33, 85.99, 73.65 |
Refinement procedure
| Resolution | 48.753 - 2.393 |
| R-factor | 0.1947 |
| Rwork | 0.191 |
| R-free | 0.24140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.110 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 48.753 | 50.000 | 2.490 |
| High resolution limit [Å] | 2.390 | 5.170 | 2.390 |
| Number of reflections | 39310 | ||
| Completeness [%] | 99.0 | 100 | 96.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 10mM Hepes, 20mM NaCl, 2mM MgCl2, 0.5 mM DTT, 2mM NaN3, 0.2 mM EGTA, 30% MME PEG 2K, 10% MPD, pH 7.0, VAPOR DIFFUSION, SITTING DROP |
