3TRZ
Mouse Lin28A in complex with let-7d microRNA pre-element
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-09-08 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 143.820, 143.820, 177.720 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 66.660 - 2.900 |
| R-factor | 0.1847 |
| Rwork | 0.183 |
| R-free | 0.21020 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.300 |
| Data reduction software | XDS (autoPROC) |
| Data scaling software | SCALA |
| Phasing software | BUSTER (2.11.1) |
| Refinement software | BUSTER-TNT (BUSTER 2.11.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.660 | 2.910 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.071 | 0.586 |
| Number of reflections | 41278 | |
| <I/σ(I)> | 21.4 | 3.2 |
| Completeness [%] | 98.5 | 99.8 |
| Redundancy | 5.6 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 298 | 0.6M NaH2PO4, 1.4M K2HPO4, pH 7, vapor diffusion, temperature 298K |
