3TRT

Crystal structure of stabilised vimentin coil2 fragment

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	CCD
Collection date	2010-03-27
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9793
Spacegroup name	I 2 2 2
Unit cell lengths	53.419, 75.521, 86.859
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.034 - 2.300
R-factor	0.2336
Rwork	0.225
R-free	0.29840
Structure solution method	MAD
RMSD bond length	0.008
RMSD bond angle	1.034
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELX
Refinement software	PHENIX (dev_780)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	28.000	28.000	2.340
High resolution limit [Å]	2.300	6.120	2.300
Number of reflections	14768
<I/σ(I)>	10.7		2
Completeness [%]	97.8	99.4	87.1
Redundancy	3.4	3.6	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	298	2M ammonium sulphate, 0.1M Tris pH8.5 + protein in 10 mM Tris pH 8, 38 mM NaCl, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	8.5	298	2M ammonium sulphate, 0.1M Tris pH8.5 + protein in 10 mM Tris pH 8, 38 mM NaCl, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	8.5	298	2M ammonium sulphate, 0.1M Tris pH8.5 + protein in 10 mM Tris pH 8, 38 mM NaCl, VAPOR DIFFUSION, HANGING DROP, temperature 298K