3TR8
Structure of an oligoribonuclease (orn) from Coxiella burnetii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-05-17 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 117.656, 61.300, 58.094 |
| Unit cell angles | 90.00, 112.41, 90.00 |
Refinement procedure
| Resolution | 26.025 - 2.503 |
| R-factor | 0.2105 |
| Rwork | 0.207 |
| R-free | 0.27550 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 0.725 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.7_650) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.540 |
| High resolution limit [Å] | 2.500 | 6.780 | 2.500 |
| Rmerge | 0.076 | 0.059 | 0.167 |
| Number of reflections | 13189 | ||
| <I/σ(I)> | 8.1 | ||
| Completeness [%] | 98.3 | 96.5 | 76.1 |
| Redundancy | 3.6 | 3.5 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 295 | 0.08M sodium cacodylate, 0.16M magnesium acetate, 16% PEG 8000, 20% glycerol, pH 6.5, vapor diffusion, sitting drop, temperature 295K |
