3TQY
Structure of a single-stranded DNA-binding protein (ssb), from Coxiella burnetii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4C |
| Synchrotron site | NSLS |
| Beamline | X4C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-08-26 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.842, 103.702, 54.995 |
| Unit cell angles | 90.00, 94.78, 90.00 |
Refinement procedure
| Resolution | 37.178 - 2.600 |
| R-factor | 0.2071 |
| Rwork | 0.203 |
| R-free | 0.27500 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.059 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.7_650) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.640 |
| High resolution limit [Å] | 2.600 | 7.050 | 2.600 |
| Rmerge | 0.079 | 0.070 | 0.364 |
| Number of reflections | 19173 | ||
| <I/σ(I)> | 9.4 | ||
| Completeness [%] | 98.6 | 98.8 | 87.1 |
| Redundancy | 7.2 | 7.4 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 295 | 0.1 HEPES pH 7.0 5% PEG 6000, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
