3TQQ
Structure of the methionyl-tRNA formyltransferase (fmt) from Coxiella burnetii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.820, 56.295, 62.868 |
| Unit cell angles | 90.00, 113.66, 90.00 |
Refinement procedure
| Resolution | 17.842 - 2.000 |
| R-factor | 0.1982 |
| Rwork | 0.196 |
| R-free | 0.24600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.957 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.6.4_486) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.086 |
| High resolution limit [Å] | 2.000 | 6.730 | 2.000 |
| Rmerge | 0.106 | 0.069 | 0.285 |
| Number of reflections | 21868 | ||
| <I/σ(I)> | 13.4 | ||
| Completeness [%] | 96.0 | 97.9 | 84.9 |
| Redundancy | 3.4 | 3.5 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 200 mM Ammonium Sulfate, 20% PEG 3350, pH 7.5, vapor diffusion, sitting drop, temperature 293K |
