3TQL
Structure of the amino acid ABC transporter, periplasmic amino acid-binding protein from Coxiella burnetii.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4C |
| Synchrotron site | NSLS |
| Beamline | X4C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-07-28 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 46.421, 66.450, 87.389 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.694 - 1.594 |
| R-factor | 0.164 |
| Rwork | 0.161 |
| R-free | 0.21480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.077 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.6.4_486) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.630 |
| High resolution limit [Å] | 1.594 | 4.340 | 1.600 |
| Rmerge | 0.075 | 0.043 | 0.371 |
| Number of reflections | 35438 | ||
| <I/σ(I)> | 13.4 | ||
| Completeness [%] | 97.1 | 100 | 99.9 |
| Redundancy | 4.3 | 8.8 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | sitting drop | 7.5 | 293 | 20% PEG 6000, 100 mM Hepes, pH 7.5, sitting drop, temperature 293K |
