3TQ1

Human DNA Polymerase eta in binary complex with DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2011-02-14
Detector	ADSC QUANTUM 315
Wavelength(s)	1.00
Spacegroup name	P 61
Unit cell lengths	98.914, 98.914, 81.185
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.270 - 2.556
R-factor	0.19086
Rwork	0.186
R-free	0.24801
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.034
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.5.0102)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.640
High resolution limit [Å]	2.550	2.550
Number of reflections	14698
<I/σ(I)>	14.6
Completeness [%]	99.8	99.1
Redundancy	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	20%-35% PEG 3350, 0.1M Bis-Tris pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K