3TP7
Crystal structure of Staphylococcal nuclease variant Delta+PHS V99E at cryogenic temperature
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OTHER |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-04-06 |
| Detector | BRUKER APEX II |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 31.083, 60.603, 37.957 |
| Unit cell angles | 90.00, 93.61, 90.00 |
Refinement procedure
| Resolution | 37.880 - 1.900 |
| R-factor | 0.1965 |
| Rwork | 0.194 |
| R-free | 0.24640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bdc |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.489 |
| Data reduction software | XPREP (2008/2 for Windows) |
| Phasing software | PHASER (2.2.1) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 37.880 | 37.880 | 1.940 |
| High resolution limit [Å] | 1.900 | 5.230 | 1.900 |
| Rmerge | 0.035 | 0.017 | 0.316 |
| Number of reflections | 11162 | 561 | 510 |
| <I/σ(I)> | 20.81 | ||
| Completeness [%] | 100.0 | 99.6 | 100 |
| Redundancy | 13.59 | 20.06 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 22% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
