3TP6
Crystal structure of Staphylococcal nuclease variant Delta+PHS L36E/L103K at cryogenic temperature
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OTHER |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-05-20 |
| Detector | BRUKER APEX II |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 31.178, 60.472, 38.063 |
| Unit cell angles | 90.00, 93.85, 90.00 |
Refinement procedure
| Resolution | 37.980 - 1.800 |
| R-factor | 0.1807 |
| Rwork | 0.179 |
| R-free | 0.21690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bdc |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.509 |
| Data reduction software | XPREP (2008/2 for Windows) |
| Phasing software | PHASER (2.2.1) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 60.470 | 60.470 | 1.840 |
| High resolution limit [Å] | 1.800 | 4.970 | 1.800 |
| Rmerge | 0.023 | 0.010 | 0.170 |
| Number of reflections | 13167 | 661 | 658 |
| <I/σ(I)> | 31.62 | ||
| Completeness [%] | 100.0 | 99.8 | 100 |
| Redundancy | 11.93 | 20.6 | 4.75 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 22% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
