3TOV
The crystal structure of the glycosyl transferase family 9 from Veillonella parvula DSM 2008
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-04-09 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97918, 0.97932 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 78.708, 95.394, 121.494 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.791 - 2.983 |
| R-factor | 0.176 |
| Rwork | 0.171 |
| R-free | 0.26690 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.176 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXD |
| Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.000 | 3.050 |
| High resolution limit [Å] | 2.983 | 3.000 |
| Rmerge | 0.136 | 0.601 |
| Number of reflections | 18816 | |
| <I/σ(I)> | 15.9 | 2.5 |
| Completeness [%] | 98.4 | 98.9 |
| Redundancy | 5.5 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | 1.6M Magnesium sulfate, 0.1M MES:NaOH., pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
