3TO1
Two surfaces on Rtt106 mediate histone binding and chaperone activity
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Detector technology | CCD |
| Collection date | 2007-08-22 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.11587 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.977, 60.703, 76.411 |
| Unit cell angles | 90.00, 104.52, 90.00 |
Refinement procedure
| Resolution | 23.227 - 2.600 |
| R-factor | 0.212 |
| Rwork | 0.209 |
| R-free | 0.25740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | model not deposited |
| RMSD bond length | 0.012 |
| RMSD bond angle | 0.845 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: dev_810)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.227 | 2.740 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 19832 | |
| Completeness [%] | 99.8 | 99.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 100mM Hepes pH 7.5, 10% PEG6000, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
